Archives of Biological Sciences 2011 Volume 63, Issue 4, Pages: 933-942
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Mesenchymal stem cell properties of dental pulp cells from deciduous teeth
Nikolić N., Krstić A., Trivanović D., Mojsilović S., Kocić J., Santibanez J.F., Jovčić G., Bugarski D.
In the present study we have isolated and identified mesenchymal stem cells
(MSCs) from the exfoliated deciduous teeth dental pulp (DP-MSCs), as
plastic-adherent, spindle-shaped cells with a high proliferative potential.
Immunophenotype analyses revealed that DP-MSCs were positive for mesenchymal
cell markers (CD90, CD44, CD105, STRO-1, vimentin and α-SMA), and negative
for hematopoietic stem cell markers (CD11b, CD33, CD34, CD45, CD235a). DPMSCs
were also capable of differentiating into adipogenic, chondrogenic, myogenic
and osteogenic lineages, fulfilling the functional criterion for their
characterization. These results demonstrate that DP-MSCs offer a valuable,
readily accessible source to obtain and store adult stem cells for future
Keywords: Dental pulp, deciduous teeth, mesenchymal stem cells, CFU-F, growth, differentiation