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Hemijska industrija 2002 Volume 56, Issue 9, Pages: 386-391
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Fractionation of the mixture obtained by the enzymatic hydrolysis of penicillin G

Cascaval Dan, Oniscu Corneliu, Galaction Anca-Irina

Using the experimental data on the individual physical and reactive extraction of 6APA with 1,2-dichlororethane, D2EHPA and Amberlite LA-2 together with data on the influence of the pH value of the aqueous phase and the Amberlite LA-2 concentration on the selective separation of 6APA, PG and PAA from a mixture, a process flow sheet for the fractionation of the solution obtained by the enzymatic hydrolysis of PG has been elaborated and applied. Thus, at pH=10, 6APA was selectively separated by four extraction stages, the overall extraction degree being 98.8%. PG was selectively extracted from the raffinate at pH=6 by three extraction stages, adjusting the molar concentration of Amberlite LA-2 to the value of the PG molar concentration for each extraction stage. The antibiotic extraction degree was 99.6%.

Keywords: reactive extraction, 6-aminopenicillanic acid, penicillin G, phenylacetic acid, amberlite LA-2, di-(2-ethylhexyl) phosphoric acid

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