Genetika 2008 Volume 40, Issue 1, Pages: 9-16
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The main seed storage proteins among high-protein soybean genotypes
Taški-Ajduković Ksenija, Đorđević Vuk, Vidić Miloš, Vujaković Milka, Milosević Mirjana, Miladinović Jegor
It is known that the main components of the seed storage proteins contribute to the quality of soybean [Glycine max (L.) Merr.] food products. The objective of this study was to investigate content of the two of them [glycinin (11S) and β-conglycinin (7S) fractions] and their respective subunits on the new high-protein soybean genotypes from the Institute for Field and Vegetable Crops, Novi Sad, Serbia. Subunits were resolved by SDS-PAGE and gels were analyzed by scanning densitometry. Out of 20 analyzed genotypes, the β' and β subunits of β- conglycinin were significantly higher in all of the genotypes except KO531 and KO5431. The β subunit of β-conglycinin was significantly higher in genotypes KO535 KO5437, KO534, KO537, KO539, KO5439, KO532, KO5435, KO538, KO5438 and KO533. The acetic polypeptides of glycinin were significantly higher in genotypes KO5439, KO5437, KO5436, KO5438, KO5432, KO5435, KO5433 and KO5434. The basic polypeptides of glycinin were not significantly higher only in genotypes KO539, KO536, KO538, KO535 and KO533. In conclusion, it appears that among the new high-protein genotypes there are genotypes with different amount of subunits that should be bread in the future for a desired level of the protein components. .
Keywords: Glycine max, β-conglycinin, glycinin, high-proteins seeds, soybean, storage proteins
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