Archives of Biological Sciences 2010 Volume 62, Issue 4, Pages: 1071-1081
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Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.
Živković Suzana, Popović M., Dragišić-Maksimović Jelena, Momčilović Ivana, Grubišić D.
Asplenium ceterach belongs to a group of poikilohydric ferns and it can
recover uninjured from an almost completely dehydrated state. In our study,
short term dehydration (24h) at four different water potentials, resulted in
moderate water loss (partial desiccation) in fern tissue. The main phenolic
acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA)
and their content decreased during the dehydration process. For the first
time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined
in the rustyback fern. The results exhibit the presence of numerous anionic
POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic
isoforms was detected. Two PPO isoforms were identified, one anionic with pI
6.3 and one cationic with pI of about 9.0. Short-term dehydration brought
about a remarkable increase in POD and PPO activity using CGA as a substrate.
Changes in enzyme activity and content of substrates during dehydration may
play an important role in the adaptation of the rustyback fern to water
deficit, and increase the overall plant resistance to stress conditions.
Keywords: Asplenium ceterach, dehydration, peroxidase, polyphenol oxidase