Medicinski pregled 2007 Volume 60, Issue 3-4, Pages: 128-133
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C-fos protein expression in the parietal cortex and olfactory tubercle in the hypoxic rat brain
Puškaš Laslo, Puškaš Nela, Babović Siniša S., Velicki Lazar, Ivanov Dejan, Mijatov-Ukropina Ljilja
Introduction. We have attempted to identify which parts of the brain react to ischemic attack using the four-vessel occlusion model in rats. Material and methods. We have monitored the expression of c-fos protein in the parietal cortex (R3 and T3) and in the olfactory tubercle (R4 and T4), regions which are supplied with blood by different arteries. The four-vessel occlusion was performed using the Pulsinelli’s method and rats were divided into two groups: total ischemia (ligation of four blood vessels or coagulation of the vertebral artheries with bilateral ligation of carotid arteries - R group) and transient ischemic attack (ligation of four blood vessels or coagulation of the vertebral arteries with bilateral repeated ligation of carotid arteries - T group of rats, the so- called preconditioned group). Each of these groups had a control group. Results and conclusion. The results showed pronounced expression of c-fos neurons in T group of rats which can explain longer survival of neurons. We believe that this model can serve as a good starting point to developing new approaches to the therapy of brain ischemia.
Keywords: hypoxia, brain, rats, proto-oncogene proteins c-fos, parietal lobe, olfactory pathways
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