Hemijska industrija 2012 Volume 66, Issue 4, Pages: 547-552
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Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts
Radojković Marija M., Zeković Zoran P., Vidović Senka S., Kočar Drago D., Mašković Pavle Z.
Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been
used in traditional medicine as diuretic, hypoglycemic and hypotensive. The
mechanism of their effects is correlated with the content of active
components. Objective of this work was to evaluate and compare antioxidant
properties of different extracts of two Morus species growing in Serbia:
Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential
antioxidant activity, content of antioxidant compounds (phenolics and
flavonoids) and radical scavenging capacity, tested by DPPH method, were
evaluated. The phenolic and flavonoid composition of different Morus extracts
was determined by the HPLC method. The extracts prepared from fruits, leaves
and roots of M. alba and M. nigra exhibited different characteristics. The
highest extraction yield was achieved by M. alba leaves extraction (23.40%).
M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g),
while highest total flavonoids content (67.37 mg RE/g) was determined for M.
nigra leaves extracts. In addition, black mulberry leaves extracts with the
highest antioxidant activity had the highest phenolic acids contents. The
dominant phenolic components in the samples were rutin and chlorogenic acid.
All investigated mulberry dry extracts shown high content of phenolic
compounds and significant antioxidant activity. This work contributes to
knowledge of the antioxidant properties of Morus species. The obtained
results may be useful in the evaluation of new dietary supplements and food
Keywords: Morus nigra, Morus alba, phenols, flavonoids, HPLC