Hemijska industrija 2013 Volume 67, Issue 3, Pages: 411-418
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Quercetin oxidation by horseradish peroxidase: The effect of UV-B irradiation

Savić Saša R., Stanojević Jelena S., Marković Dejan Z., Petronijević Živomir B.

Horseradish peroxidase (HRP), a highly-investigated member of the peroxidase family has been known, among many other biological activities, to catalyze the oxidation of flavonoids and phenolic substrates overall, including quercetin. On the other hand, quercetin is very well known for its antioxidant activities, which in the case of UV external radiation is exibited partly in a preventive manner since it is an excellent UV-absorber. Therefore the aim of this investigation is to study quercetin oxidation by HRP in phosphate buffer under the conditions of UV-stress, i.e. continuous, prolonged UV-B irradiation. The results show that while UV-B irradiation affects the activity of HRP, and the overal rate of quercetin oxidation by HRP, it probably has very little effect on it for longer UV-B-irradiation periods (>30 min). [Acknowledgements. This work was supported by the Ministry of Education and Science of the Republic of Serbia under Project No.TR-34012 and OI-172044]

Keywords: quercetin, peroxidase, UV-B radiation, kinetics, oxidation