Archives of Biological Sciences 2007 Volume 59, Issue 1, Pages: 45-49
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Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

Miljuš-Đukić Jovanka D., Radović Svetlana R., Maksimović Vesna R.

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.

Keywords: Buckwheat, heteromorphic self-incompatibility, protease inhibitors

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