Jugoslovenska medicinska biohemija 2002 Volume 21, Issue 3, Pages: 275-282
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6-hydroxydopamine lesions of the striatum lead to the alterations of dopamine receptor mrna in parkinsonian rats

Mladenović Aleksandra, Perović Milka, Milanović Desanka J., Kanazir Selma D., Rakić Ljubisav, Ruždijić Sabera D.

The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.

Keywords: Parkinsonian rats, 6-OHDA lesions, behavior, D1 and D2 mRNA, RT-PCR.