Journal of the Serbian Chemical Society 2012 Volume 77, Issue 4, Pages: 437-451
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Antioxidant activities and phenolic constituents of Cephalotaxus oliveri Mast. aerial parts
Zeng Lingbin, Vrijmoed L.L.P.
The present study was to analyze the antioxidant activity and the responsible
chemical constituents in Cephalotaxus oliveri Mast. aerial parts. DPPH
(2,2-diphenyl-1-picryl hydrazyl), ABTS
[2,2’-azinobis(3-ethylbenzothiazoline- 6-sulfonic acid)], reducing power and
total phenolic content assays indicate that the methanol extract of the
aerial parts is the most potent radical-scavenger and reducing agent with
the highest level of phenolic content among the test extracts. The highly
positive linear correlations implicate that the four assays have similar
capacity to predict the antioxidant potential of the aerial parts and
phenolic compounds present contribute significantly to DPPH and ABTS radical
scavenging activity and reducing power of the extracts. The plant also
displayed considerable superoxide anion radical scavenging activity.
LC-MS/MS and GC-MS analyses resulted in the identification of 22 compounds
in the methanol extract, among which are 15 phenolic compounds. The total
amount of the phenolic compounds in the methanol extract determined by HPLC
method was more than 5622.39 μg/g dry weight. The considerable antioxidant
potential and a high content of phenolic antioxidants suggest that C.
oliveri aerial parts are a potential source of natural antioxidants.
Keywords: radical scavenging activity, reducing power, total phenolic content, LC-MS/MS, HPLC, GC-MS