Journal of the Serbian Chemical Society 2004 Volume 69, Issue 11, Pages: 871-875
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Allosteric regulation of small-molecule binding by aptimers
Stojanović Milan N.
Modular aptameric sensors for oligonucleotides were constructed by combiningmolecular beacon stem-loops andmalachite green aptamers. These sensorswere designed so that oligonucleotides complementary to the stem-loop region accomplish the release of malachite green from its binding module. The oligonucleotides that bound to both loop and stem caused efficient release of the dye. While this system is not suitable for single-mismatch detection, it represents proof-of-concept for drug delivery components of autonomous therapeutic devices on a molecular scale.
Keywords: aptamers, allosteric control, fluorescence, malachite