Archives of Biological Sciences 2010 Volume 62, Issue 4, Pages: 941-945
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Antibacterial and free-radical-scavenging properties of Stachys schtschegleevii (Lamiaceae)
Abichandani Malvika, Nahar Lutfun, Singh Poonam, Chitnis Rohit, Nazemiyeh H., Delazar A., Sarker S.D.
Stachys schtschegleevii Sosn. (Lamiaceae alt. Labiatae) is one of 34 Stachys
species found in Iran, and is morphologically similar to Stachys inflata.
This plant has been used in the Iranian traditional medicine as a remedy for
bacterial infections, rheumatic fever and other inflammatory conditions. The
n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of the
non-flowering aerial parts of this plant were assessed for their
antibacterial and freeradical- scavenging activities using the
micro-titer-based antimicrobial assay incorporating resazurin as an indicator
of cell growth and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay,
respectively. While the n-hexane extract did not show any
free-radical-scavenging activity, the MeOH extract showed the highest level
of activity with a RC50 value of 2.94 x 10-2 mg/mL, which was about ten-fold
less active than the positive control Trolox® (RC50 = 2.60 x 10-3 mg/mL).
None of the extracts showed any antibacterial property against Bacillus
cereus and B. subtilis at test concentrations. However, all extracts were
active against ampicillin-resistant Escherichia coli and Staphylococcus
aureus. The MeOH extract was the most potent (MIC range 1.56-6.25 mg/mL)
among the extracts and was most active against ampicillin-resistant E. coli
(MIC = 1.56 mg/mL).
Keywords: Stachys schtschegleevii, Lamiaceae, antibacterial activity, free-radical-scavenger, activity, resazurin assay, DPPH assay
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