Biotechnology in Animal Husbandry 2004 Volume 20, Issue 3-4, Pages: 49-54
doi:10.2298/BAH0404049R
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Lactic acid bacteria strains isolated from Sjenica cheese

Radulović Zorica, Martinović Aleksandra, Radin Dragoslava D., Obradović D.

Lactic acid bacteria strains isolated from autochthonous cheeses of Sjenica were investigated. In those products, where starters were not added fermentation occurred as a result of natural flora present in the surrounding environment. Forty thermophilic, mesophilic and citrat+ LAB strains were isolated by selective mediums (MRS agar, LM17 agar and LDC agar) from 5 samples of Sjenica-cheeses. They were exposed to further analysis and identification. After examination by Gram test and catalase test, 13 strains of LAB, were selected for further analysis. On the basis of lactose fermentation way and acid production in 1% reconstituted skimed milk, 6 strains were selected. Determination of these strains by API 50 CHL and Rapid ID 32 Strep tests showed that 2 strains belonged to the Lactococcus lactis ssp. lactis, 2 strains to the Lactococcus lactis ssp. cremoris and 2 strains to the Lactobacillus para.paracasei.

Keywords: lactic acid bacteria, acidogenuos ability, Sjenica cheese

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