Hemijska industrija 2003 Volume 57, Issue 12, Pages: 600-604
doi:10.2298/HEMIND0312600P
Full text ( 516 KB)
Cited by


Characterization of agarose as immobilization matrix model for a microbial biosensor

Pernetti Mimma, Poncelet Denis, Thouand Gerald, Annesini Maria Cristina, Merli Carlo

Microbial biosensors are promising tools for the detection of specific substances in different fields, such as environmental, biomedical, food or agricultural. They allow rapid measurements, no need for complex sample preparation or specialized personnel and easy handling. In order to enhance the managing, miniaturization and stability of the biosensor and to prevent cell leaching, bacteria immobilization is desirable. A systematic characterization procedure to choose a suitable immobilization method and matrix, was proposed in this study. Physical properties, storage stability mass transport phenomena and biocompatibility were evaluated, employing agarose as the model matrix. Preliminary essays with bioluminescent bacteria detecting Tributyltin were also carried out.

Keywords: Agarose, Biosensor, Immobilization, Luminescence

More data about this article available through SCIndeks