doiSerbia

Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction

Miljković-Selimović Biljana (School of Medicine, Niš + Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, Niš)
Babić Tatjana (Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, Niš)
Kocić Branislava (School of Medicine, Niš + Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, Niš)
Matkić Aleksandra (School of Medicine, Niš)
Ristić Ljiljana (Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, Niš)

Introduction. Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. Objective. Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. Methods. A total of 153 species of thermophilic campylobacters were examined with standard biochemical tests and PCR technique to prove hipO genes of C. jejuni and asp genes of C. coli. Results Standard biochemical tests enabled the speciation of 121 strains of C. jejuni, while application of PCR detected 126 C. jejuni strains. Conclusion. PCR technique allowed not only identification of hippurate-positive C. jejuni, but also hipuratnegative strains of C. jejuni which otherwise would be detected as C. coli if only biochemical tests were applied.

Keywords: Campylobacter jejuni, Campylobacter coli, identification, PCR